EK1314

Human Endothelin 1/ET-1 Competitive ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,900.00 – ¥3,200.00

分子靶点：EDN1, endothelin 1, ET1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(原液)
灵敏度：0.11 pg/mL
检测范围：0.16 pg/mL - 20 pg/mL
回收率：90% - 108%

在售SKU:70-EK1314-48, 70-EK1314-96

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库存号

描述

文章目录[隐藏]

分子信息
EDN1 分子靶点信息概述
EDN1 分子靶点综述
- 人 Human EDN1 分子靶点信息
- 人 Human EDN1靶点分子功能（预测）

商品名	Human Endothelin 1/ET-1 Competitive ELISA Kit (人内皮素1 (ET-1) ELISA竞争法试剂盒)
检测方法	竞争法
精密度	板内变异系数：3.1% - 6.2%；板间变异系数：4.2%-7.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(原液)
灵敏度	0.11 pg/mL
检测范围	0.16 pg/mL - 20 pg/mL
回收率	90% - 108%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗ET-1单克隆抗体的96孔聚苯乙烯酶标板人ET-1冻干标准品 Angiotensinogen/AGT/ SerpinA8检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用固相竞争酶联免疫吸附检测技术。特异性抗人ET-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入生物素化的ET-1和未标记的ET-1或样本，竞争结合包被抗体上有限的结合位点。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中ET-1的浓度成反比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Endothelin 1/ET-1 Competitive ELISA Kit (人内皮素1 (ET-1) ELISA竞争法试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 EDN1, endothelin 1, ET1 靶点信息

EDN1 分子靶点信息概述

分子名：EDN1, endothelin 1
基因家族：Endothelins
别名：ET1

EDN1 分子靶点综述

内皮素-1(ET-1)是由21个氨基酸残基组成的多肽，是一种多效性分，它最初是212个氨基酸的前多肽原。血管内皮细胞是ET-1的丰富来源。内皮细胞中的ET-1的表达受多种因素的影响，包括机械刺激、多种激素和促炎性细胞因子。内皮素首先被鉴定为内皮素衍生的舒张因子，然后又被鉴定为内皮素衍生的收缩因子。ET-1刺激心肌收缩和心肌细胞生长，调节血管活性物质的释放，刺激平滑肌细胞有丝分裂。它也作为内皮细胞的促生因子，由下丘脑和垂体细胞调节其分泌。ET-1可能通过促进中性粒细胞的粘附和迁移以及刺激促炎细胞因子的产生来控制炎症反应。

人 Human EDN1 分子靶点信息

分子名：EDN1, endothelin 1
别称：
- ARCND3
- endothelin-1
- ET1
- HDLCQ7
- PPET1
- preproendothelin-1
- QME
基因序列：NCBI_Gene: 1906
蛋白序列：UniProtKB: P05305

人 Human EDN1靶点分子功能（预测）

Enables signaling receptor binding activity. Involved in several processes, including intracellular signal transduction; regulation of macromolecule biosynthetic process; and vascular associated smooth muscle contraction. Located in cytoplasm and extracellular space. Implicated in several diseases, including artery disease (multiple); bronchial disease (multiple); obesity; obstructive sleep apnea; and rheumatoid arthritis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); glaucoma (multiple); lung disease (multiple); and retinal disease (multiple).