EK1F03

Human FGF basic ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：FGF2, FGFB
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.39 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：92% - 119%

在售SKU:70-EK1F03-48, 70-EK1F03-96

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库存号

描述

文章目录[隐藏]

分子信息
FGF2 分子靶点信息概述
FGF2 分子靶点综述
- 人 Human FGF2 分子靶点信息
- 人 Human FGF2靶点分子功能（预测）

商品名	Human FGF basic ELISA Kit (人碱性成纤维生长因子 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.8% - 6.5%；板间变异系数：5.3% - 10.2%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.39 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	92% - 119%
平均回收率	1.1
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗FGF basic单克隆抗体的96孔聚苯乙烯酶标板人FGF basic冻干标准品 FGF basic检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(22×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人FGF basic抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的FGF basic与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中FGF basic的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human FGF basic ELISA Kit (人碱性成纤维生长因子 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 FGF2, FGFB 靶点信息

FGF2 分子靶点信息概述

分子名：FGF2, fibroblast growth factor 2
基因家族：Receptor ligands; Fibroblast growth factor family
曾用名：FGFB
全称：fibroblast growth factor 2 (basic)

FGF2 分子靶点综述

碱性成纤维细胞生长因子(FGFbasic)，又名bFGF、FGF2或FGF-β，是成纤维细胞生长因子家族中的一员。在正常组织的伤口愈合和肿瘤发展过程中，硫酸乙酰肝素降解酶激活FGFbasic来介导血管生成。另外，人类脂肪细胞可合成和分泌FGFbasic，它的浓度和血液样本中的BMI相关。研究显示低水平的FGFbasic在过度焦虑的发生中发挥重要作用。此外，FGFbasic是人类胚胎干细胞培养基中的重要组成成分，是维持细胞未分化状态的必要因素。FGFbasic与BMP4一起可促进干细胞分化为中胚层谱系。相比于未处理的干细胞，经BMP4和FGFbasic处理过的干细胞通常会分化出更多的成骨细胞和软骨细胞。

人 Human FGF2 分子靶点信息

分子名：FGF2, fibroblast growth factor 2
别称：
- basic fibroblast growth factor bFGF
- BFGF
- FGF-2
- FGFB
- fibroblast growth factor 2 (basic)
- HBGF-2
- heparin-binding growth factor 2
- prostatropin
基因序列：NCBI_Gene: 2247
蛋白序列：UniProtKB: P09038

人 Human FGF2靶点分子功能（预测）

Enables several functions, including identical protein binding activity; nuclear receptor coactivator activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and regulation of cell migration. Acts upstream of or within positive regulation of cell fate specification and positive regulation of cell population proliferation. Located in extracellular space. Implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Biomarker of several diseases, including artery disease (multiple); breast cancer (multiple); carcinoma (multiple); eye disease (multiple); and female reproductive organ cancer (multiple).