EK112

Human IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

  • 分子靶点：IL12, IL-12, CLMF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
  • 灵敏度：0.32 pg/mL
  • 检测范围：1.56 pg/mL - 100 pg/mL
  • 回收率：84% - 119%

在售SKU:70-EK112-48, 70-EK112-96, 人, 白介素12, 白介12, IL12, interleukin 12

¥<\/span>1,600.00<\/bdi><\/span><\/span>","sku":"70-EK112\/2-48","variation_description":"","variation_id":8088,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":2650,"display_regular_price":2650,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>2,650.00<\/bdi><\/span><\/span>","sku":"70-EK112\/2-96","variation_description":"","variation_id":8089,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：5.5% - 7.5%；板间变异系数：3.2% - 7.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：10 μL；细胞培养上清：100 μL
灵敏度

0.32 pg/mL
检测范围

1.56 pg/mL - 100 pg/mL
回收率

84% - 119%
平均回收率

1.03
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-12p70单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-12p70冻干标准品
  • IL-12p70检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒) - 标准曲线
标准曲线
IL12A 分子靶点信息概述IL12B 分子靶点信息概述

IL12A 分子靶点信息概述

  • 分子名：IL12A, interleukin 12A
  • 基因家族：Interleukins
  • 别名：CLMF; IL-12A; p35; NFSK
  • 曾用名：NKSF1
  • 全称：nterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL35 subunit

IL12B 分子靶点信息概述

  • 分子名：IL12B, interleukin 12B
  • 基因家族：Immunoglobulin like domain containing; Interleukins
  • 别名：CLMF; IL-12B; NKSF; CLMF2
  • 曾用名：NKSF2
  • 全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

IL12, interleukin 12 分子靶点种属信息

人 Human IL1A人 Human IL1B

人 Human IL12A 分子靶点信息

  • 分子名：IL12A, interleukin 12A
  • 别称：
    • CLMF
    • CLMF p35
    • cytotoxic lymphocyte maturation factor 1, p35
    • cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12, subunit p35
    • IL-12A
    • IL35 subunit
    • interleukin 12, p35
    • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
    • interleukin-12 alpha chain
    • interleukin-12 subunit alpha
    • natural killer cell stimulatory factor 1, 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
  • 基因序列：NCBI_Gene: 3592
  • 蛋白序列：UniProtKB: P29459

人 Human IL12A靶点分子功能（预测）

Enables interleukin-12 beta subunit binding activity; interleukin-27 binding activity; and protein heterodimerization activity. Contributes to cytokine activity and growth factor activity. Involved in several processes, including positive regulation of leukocyte mediated cytotoxicity; positive regulation of lymphocyte proliferation; and response to bacterium. Acts upstream of or within negative regulation of protein secretion. Located in extracellular space. Part of interleukin-12 complex. Implicated in hepatitis C; hepatocellular carcinoma; and primary biliary cholangitis. Biomarker of hepatitis C.

人 Human IL12B 分子靶点信息

  • 分子名：IL12B, interleukin 12B
  • 别称：
    • CLMF
    • CLMF p40
    • CLMF2
    • cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL-12 subunit p40
    • IL-12B
    • IL12, subunit p40
    • IMD28
    • IMD29
    • interleukin 12, p40
    • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2
    • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
    • interleukin-12 beta chain
    • interleukin-12 subunit beta
    • natural killer cell stimulatory factor, 40 kD subunit
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF2
  • 基因序列：NCBI_Gene: 3593
  • 蛋白序列：UniProtKB: P29460

人 Human IL12B靶点分子功能（预测）

Enables identical protein binding activity; interleukin-12 alpha subunit binding activity; and protein heterodimerization activity. Contributes to cytokine activity; growth factor activity; and interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of lymphocyte proliferation; positive regulation of peptidyl-tyrosine phosphorylation; and regulation of cytokine production. Acts upstream of or within negative regulation of protein secretion. Part of interleukin-12 complex and interleukin-23 complex. Implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Biomarker of anogenital venereal wart.

操作步骤

下载本产品说明书

ELISA操作常见问题

五颜六色的真空采血管，你用对了吗？ 

直播预告 | 实验太繁琐？蛋白浓度低？用一步法和高敏型ELISA 

ELISA系列 | ELISA检测TGF-β，样本该怎样活化？ 

血清OR血浆，哪个是ELISA的菜 

ELISA系列 | 血管内皮生长因子（VEGF） 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

直播预告 | ELISA实验技能要点与常见问题解析 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

  1. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  2. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  3. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  4. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  5. 血清

    Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

评价

目前还没有评价

成为第一个“Human IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注

相关产品