EK1208

Human TRANCE/TNFSF11/RANKL ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：TNFSF11, RANKL, CD254
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：0.75 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：80% - 102%

在售SKU:70-EK1208-48, 70-EK1208-96

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库存号

描述

文章目录[隐藏]

分子信息
TNFSF11 分子靶点信息概述
TNFSF11 分子靶点综述
- 人 Human TNFSF11 分子靶点信息
- 人 Human TNFSF11靶点分子功能（预测）

商品名	Human TRANCE/TNFSF11/RANKL ELISA Kit (人 TRANCE/TNFSF11/RANKL ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：5.6% - 8.6%；板间变异系数：2.2% - 7.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	0.75 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	80% - 102%
平均回收率	0.91
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TRANCE/TNFSF11/RANKL单克隆抗体的96孔聚苯乙烯酶标板人TRANCE/TNFSF11/RANKL冻干标准品 TRANCE/TNFSF11/RANKL检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TRANCE抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的TRANCE与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TRANCE的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human TRANCE/TNFSF11/RANKL ELISA Kit (人 TRANCE/TNFSF11/RANKL ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 TNFSF11, RANKL, CD254 靶点信息

TNFSF11 分子靶点信息概述

分子名：TNFSF11, TNF superfamily member 11
基因家族：CD molecules; Tumor necrosis factor superfamily
别名：TRANCE; RANKL; OPGL; ODF; CD254
全称：tumor necrosis factor (ligand) superfamily, member 11

TNFSF11 分子靶点综述

肿瘤坏死因子相关激活诱导因子(TRANCE)，又名肿瘤坏死因子配体超家族成员11(TNFSF11)、核因子κB受体活化因子配基(RANKL)，是TNF超家族成员。成骨细胞、CD4+和CD8+T细胞、巨核细胞、支气管和肠道上皮细胞等都可表达TRANCE。TRANCE在免疫应答和骨形态发生中起核心作用。在骨形成和重塑过程中，成骨细胞表达的TRANCE与M-CSF一起诱导破骨细胞形成和活化，导致骨吸收，进而导致随后的骨沉积和重塑。TRANCE同样在病理性骨质疏松中起作用。活化的淋巴细胞和成骨细胞不恰当表达TRANCE就会导致关节和骨头的过度吸收与沉积。过量的TRANCE与许多退行性骨疾病，如类风湿性关节炎、银屑病性关节炎有关。

人 Human TNFSF11 分子靶点信息

分子名：TNFSF11, TNF superfamily member 11
别称：
- CD254
- hRANKL2
- ODF
- OPGL
- OPTB2
- osteoclast differentiation factor
- osteoprotegerin ligand
- RANKL
- receptor activator of nuclear factor kappa B ligand
- receptor activator of nuclear factor kappa-B ligand
- sOdf
- TNF-related activation-induced cytokine
- TNLG6B
- TRANCE
- tumor necrosis factor (ligand) superfamily member 11
- tumor necrosis factor (ligand) superfamily, member 11
- tumor necrosis factor ligand 6B
- tumor necrosis factor ligand superfamily member 11
- tumor necrosis factor superfamily member 11
基因序列：NCBI_Gene: 8600
蛋白序列：UniProtKB: O14788

人 Human TNFSF11靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including positive regulation of MAPK cascade; positive regulation of cell-cell adhesion; and regulation of transcription, DNA-templated. Predicted to be located in extracellular region and plasma membrane. Implicated in autosomal recessive osteopetrosis 2 and osteoporosis. Biomarker of myocardial infarction.