EK2216

Mouse CCL22/MDC ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：CCL22, MDC, SCYA22, ABCD-1
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：80 μL 1×检测缓冲液和20 μL样本
灵敏度：4.07 pg/mL
检测范围：31.25 pg/mL - 2,000 pg/mL
回收率：76% - 92%

在售SKU:70-EK2216/2-48, 70-EK2216/2-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
CCL22 分子靶点信息概述
CCL22 分子靶点综述
- 小鼠 Mouse Ccl22 分子靶点信息
- 小鼠 Mouse Ccl22 靶点分子功能（预测）

商品名	Mouse CCL22/MDC ELISA Kit (小鼠趋化因子配体22/巨噬细胞来源的趋化因子 (MDC) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.4% - 5.1%；板间变异系数：3.6% - 5.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	80 μL 1×检测缓冲液和20 μL样本
灵敏度	4.07 pg/mL
检测范围	31.25 pg/mL - 2,000 pg/mL
回收率	76% - 92%
平均回收率	0.84
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Mouse CCL22/MDC单克隆抗体的96孔聚苯乙烯酶标板 Mouse CCL22/MDC冻干标准品 Mouse CCL22/MDC检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL22抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CCL22与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL22的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CCL22/MDC ELISA Kit (小鼠趋化因子配体22/巨噬细胞来源的趋化因子 (MDC) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 CCL22, MDC, SCYA22, ABCD-1 靶点信息

CCL22 分子靶点信息概述

分子名：CCL22, C-C motif chemokine ligand 22
基因家族：Chemokine ligands
别名：MDC; STCP-1; ABCD-1; DC/B-CK; A-152E5.1; MGC34554
曾用名：SCYA22
全称：small inducible cytokine subfamily A (Cys-Cys), member 22; chemokine (C-C motif) ligand 22

CCL22 分子靶点综述

趋化因子配体22 (CCL22)，又名巨噬细胞来源的趋化因子(MDC)，是CC趋化因子家族中的一员。B细胞、小胶质细胞、树突状细胞、巨噬细胞、单核细胞、干扰素γ激活的角质细胞、结肠上皮细胞、CD4+ T细胞、胸腺髓质上皮细胞(小体)和活化的NK细胞都可表达CCL22。 CCL22对淋巴细胞迁移有重要作用，它是树突状细胞、胸腺细胞和活化的NK细胞等细胞类型的有效趋化物。抗原递呈树突状细胞产生的CCL22趋化携有CCR4的活化 (或记忆)T细胞，从而增强机体的免疫应答，增加效应功能。CCL22还有其他功能，包括血小板功能的激活、抑制海马神经元之间的突触传递。

小鼠 Mouse Ccl22 分子靶点信息

分子名：Ccl22, chemokine (C-C motif) ligand 22
别称：
- ABCD-1
- MDC
- Scya22
- small inducible cytokine subfamily A22
基因序列：NCBI_Gene: 20299
蛋白序列：

小鼠 Mouse Ccl22 靶点分子功能（预测）

Predicted to enable CCR chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of ERK1 and ERK2 cascade. Predicted to act upstream of or within chemotaxis. Located in extracellular space. Is expressed in inguinal lymph node; thymus; and thymus primordium. Orthologous to human CCL22 (C-C motif chemokine ligand 22).