EK397

Rat Leptin ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,200.00 – ¥3,700.00

分子靶点：LEP, OBS, OB
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：1.14 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：92% - 130%

在售SKU:70-EK397-48, 70-EK397-96 大鼠瘦素大鼠瘦素

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库存号

描述

文章目录[隐藏]

分子信息
LEP 分子靶点信息概述
LEP 分子靶点综述
- 大鼠 Rat Lep 分子靶点信息
- 大鼠 Rat Lep 靶点分子功能（预测）

商品名	Rat Leptin ELISA Kit (大鼠瘦素 (Leptin) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.5% - 4.6%；板间变异系数：4.2% - 4.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	1.14 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	92% - 130%
平均回收率	1.07
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗 Leptin单克隆抗体的96孔聚苯乙烯酶标板大鼠 Leptin冻干标准品 Leptin检测抗体检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠Leptin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Leptin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Leptin的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat Leptin ELISA Kit (大鼠瘦素 (Leptin) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 LEP, OBS, OB 靶点信息

LEP 分子靶点信息概述

分子名：LEP, leptin
基因家族：Neuropeptides
曾用名：OBS; OB
全称：leptin (murine obesity homolog); leptin (obesity homolog, mouse)

LEP 分子靶点综述

瘦素是由脂肪细胞分泌的蛋白质类激素，可通过抑制饥饿来调控能量平衡。除了白色脂肪组织可以分泌瘦素外，褐色脂肪、胎盘、卵巢、骨骼肌、胃、乳腺上皮细胞、骨髓和P/D1细胞也能产生瘦素。瘦素和饥饿激素都作用于下丘脑弓状核的受体，以此来调节食欲，获得能量平衡。瘦素的基本功能是调节脂肪储存，但它在其它生理代谢过程中同样发挥作用，然而许多其它功能还未明确。研究表明瘦素与肥胖和减肥有关联。

大鼠 Rat Lep 分子靶点信息

分子名：Lep, leptin
别称：
- OB
- obese
- obesity (murine homolog leptin)
- obesity factor
基因序列：NCBI_Gene: 25608
蛋白序列：
- UniprotKB: A0A1C9CJH3
- UniprotKB: P50596

大鼠 Rat Lep 靶点分子功能（预测）

Enables hormone activity and peptide hormone receptor binding activity. Involved in several processes, including positive regulation of cell communication; regulation of apoptotic process; and response to vitamin. Located in cytosol and extracellular space. Used to study several diseases, including alcohol dependence; glucose metabolism disease (multiple); liver disease (multiple); neurodegenerative disease (multiple); and short bowel syndrome. Biomarker of several diseases, including Huntington's disease; alcohol dependence; fatty liver disease (multiple); lipid metabolism disorder (multiple); and obesity. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human LEP (leptin).