EK115

Human IL-15 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IL15
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：3.78 pg/mL
检测范围：3.13 pg/mL - 200 pg/mL
回收率：87% - 111%

在售SKU:70-EK115-48, 70-EK115-96

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库存号

描述

文章目录[隐藏]

分子信息
IL15 分子靶点信息概述
IL15 分子靶点综述
- 人 Human IL15 分子靶点信息
- 人 Human IL15靶点分子功能（预测）

商品名	Human IL-15 ELISA Kit (人白细胞介素15ELISA 试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：7.1% - 9.0%；板间变异系数：7.8% - 9.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	3.78 pg/mL
检测范围	3.13 pg/mL - 200 pg/mL
回收率	87% - 111%
平均回收率	0.97
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-15单克隆抗体的96孔聚苯乙烯酶标板人IL-15冻干标准品 IL-15 检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-15抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-15与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-15的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-15 ELISA Kit (人白细胞介素15ELISA 试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 IL15 靶点信息

IL15 分子靶点信息概述

分子名：IL15, interleukin 15
基因家族：Interleukins
别名：IL-15; MGC9721

IL15 分子靶点综述

白细胞介素15(IL-15)是一种促炎性蛋白，在中性粒细胞、树突状细胞和巨噬细胞的活化中起作用，对于NK细胞和CD8+T细胞的发育和生存是必不可少的。IL-15通过异三聚体受体信号激活Jak/Stat途径。虽然IL-15mRNA在许多细胞类型中表达，但是该分泌蛋白在生物样品中很少检测得到。最近的研究表明，IL-15滞留在胞内，只有当与其唯一的受体IL-15Rα形成复合物才能分泌到胞外。可溶性IL-15/IL-15R复合物由小鼠树突状细胞、成纤维细胞和巨噬细胞产生，它的结合活性比单独的IL-15提高10-100倍。

人 Human IL15 分子靶点信息

分子名：IL15, interleukin 15
别称：
- IL-15
- interleukin-15
- MGC9721
基因序列：NCBI_Gene: 3600
蛋白序列：UniProtKB: P40933

人 Human IL15靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).