EK981

Human/Mouse TGF-β1 ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

分子靶点：TGFB1, TGB-β1, TGFB
种属：人 (Human); 小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本

灵敏度：3.36 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：83% - 118%

在售SKU:70-EK981-48, 70-EK981-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
TGFB1 分子靶点信息概述
TGFB1 分子靶点综述
- 人 Human TGFB1 分子靶点信息
- 人 Human TGFB1靶点分子功能（预测）

商品名	Human/Mouse TGF-β1 ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	双抗夹心法
精密度	板内变异系数：2.0% - 5.3%；板间变异系数：4.1% - 6.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	人血清：活化样本80 μl + 720 μl 1×检测缓冲液小鼠血清：活化样本 20 μl + 480 μl 1×检测缓冲液人/小鼠血浆：活化样本 80 μl + 80μl 1×检测缓冲液
灵敏度	3.36 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	83% - 118%
平均回收率	0.99
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TGF-β1A单克隆抗体的96孔聚苯乙烯酶标板人/小鼠TGF-β1A冻干标准品 TGF-β1A检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/小鼠 TGF-β1 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 TGF-β1 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TGF-β1 的浓度成正比。加入终止液终止反应，在450nm 波长(参考波长570 - 630 nm)测定吸光度值。

Human/Mouse TGF-β1 ELISA Kit (人/小鼠/大鼠转化生长因子β1 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述

TGFB1 分子靶点信息概述

分子名：TGFB1, transforming growth factor beta 1
基因家族：Transforming growth factor beta family
别名：CED; TGFbeta
曾用名：TGFB; DPD1
全称：Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1 分子靶点综述

转化生长因子β1 (TGF-β1)是转化生长因子β超家族的多肽成员，具有多种细胞学功能，包括调控细胞生长、增殖、分化和凋亡。TGF-β与TGFA协同诱导转化。它也能作为负向自分泌生长因子。TGF-β活化和信号传导的失调可能会引起凋亡。很多细胞可合成TGF-β，且几乎都有特异性的受体。TGF-β1、TGF-β2和TGF-β3都通过相同的受体信号通路发挥功能。TGF-β1对调控免疫系统具有重要作用，对不同类型的细胞或不同发育阶段的细胞具有不同的活性。大部分免疫细胞 (或白细胞) 分泌TGF-β1。TGF-β1与癌症、自身免疫疾病、肝脏疾病、肾脏疾病、糖尿病、心血管疾病、哮喘、慢性阻塞性肺疾病 (COPD)和囊肿性纤维化 (CF) 等相关。

人 Human TGFB1 分子靶点信息

分子名：TGFB1, transforming growth factor beta 1
别称：
- CED
- DPD1
- IBDIMDE
- LAP
- latency-associated peptide
- prepro-transforming growth factor beta-1
- TGF-beta 1 protein
- TGF-beta-1
- TGF-beta1
- TGFB
- TGFbeta
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor beta1
基因序列：NCBI_Gene: 7040
蛋白序列：UniProtKB: P01137

人 Human TGFB1靶点分子功能（预测）

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet's disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).