EK301B

Rat IL-1β ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

分子靶点：IL1B, IL1-β, IL1F2
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：1.42 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：84% - 110%

在售SKU:70-EK301B/3-48, 70-EK301B/3-96,70-EK301B/4-48, 70-EK301B/4-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL1B 分子靶点信息概述
IL1B 分子靶点综述
- 大鼠 Rat Il1b 分子靶点信息
- 大鼠 Rat Il1b 靶点分子功能（预测）

商品名	Rat IL-1β ELISA Kit (大鼠白细胞介素1β ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：5.0% - 5.1%；板间变异系数：5.5% - 6.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	1.42 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	84% - 110%
平均回收率	1.02
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-1β单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-1β冻干标准品 IL-1β检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-1β与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-1β的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-1β ELISA Kit (大鼠白细胞介素1β ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

分子名：IL1B, interleukin 1 beta
基因家族：Interleukins
别名：IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

大鼠 Rat Il1b 分子靶点信息

分子名：Il1b, interleukin 1 beta
别称：
- IL-1 beta
- IL-1F2
- interleukin-1 beta
基因序列：NCBI_Gene: 24494
蛋白序列：
- UniprotKB: Q5BKB0
- UniprotKB: Q63264

大鼠 Rat Il1b 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).